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dc.contributor.authorErsöz, Arzu
dc.contributor.authorEmir Diltemiz, Sibel
dc.contributor.authorÖzcan, A. Atılır
dc.contributor.authorDenizli, Adil
dc.contributor.authorSay, Rıdvan
dc.date.accessioned2019-10-19T11:17:32Z
dc.date.available2019-10-19T11:17:32Z
dc.date.issued2009
dc.identifier.issn0925-4005
dc.identifier.urihttps://dx.doi.org/10.1016/j.snb.2008.12.050
dc.identifier.urihttps://hdl.handle.net/11421/11738
dc.descriptionWOS: 000264326500002en_US
dc.description.abstract8-Hydroxy-2'-deoxyguanosine (8-OHdG) is one of the most abundant oxidative DNA lesions resulting from reactive oxygen species (ROS). The routine measurement of 8-OHdG in biologic samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. In this study, we have proposed an imprinting method for 8-OHdG recognition and determination by methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] as a new metal-chelating monomer via metal coordination-chelation interactions. The study includes the solid-phase extraction of blood samples by a new 8-OHdG imprinted sorbent and the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor via ligand interaction. 8-0HdG imprinted sorbent was prepared by bulk polymerization of MAAP-Fe(III) and N-N'-methylenebisacrylamide. By using a photo-graft surface polymerization technique with irradiation by ultra-violet (UV) light, a thin MIP film was prepared for the detection of 9-OHdG. The determined affinity constant (K alpha) were found as 78760 M(-1) using 8-OHdG imprinted thin film. The 8-OHdG level in blood serum from a breast cancer patient was determined by the prepared QCM sensor. Lastly, analytical performances of this system was compared with capillary electrophoresis (CE) systemen_US
dc.description.sponsorshipThe Scientific and Technical Research Council of Turkey [TUBITAK-MAG-104M374]en_US
dc.description.sponsorshipThis study was supported by "The Scientific and Technical Research Council of Turkey" (TUBITAK-MAG-104M374).en_US
dc.language.isoengen_US
dc.publisherElsevier Science Saen_US
dc.relation.isversionof10.1016/j.snb.2008.12.050en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMolecular Imprintingen_US
dc.subjectMipen_US
dc.subjectQcmen_US
dc.subject8-Ohdgen_US
dc.title8-OHdG sensing with MIP based solid phase extraction and QCM techniqueen_US
dc.typearticleen_US
dc.relation.journalSensors and Actuators B-Chemicalen_US
dc.contributor.departmentAnadolu Üniversitesi, Bitki, İlaç ve Bilimsel Araştırmalar Merkezien_US
dc.identifier.volume137en_US
dc.identifier.issue1en_US
dc.identifier.startpage7en_US
dc.identifier.endpage11en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorErsöz, Arzu
dc.contributor.institutionauthorEmir Diltemiz, Sibel
dc.contributor.institutionauthorSay, Rıdvan


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