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dc.contributor.authorAltup, S.
dc.contributor.authorDemiryürek, A. T.
dc.contributor.authorAk, Dilek
dc.contributor.authorTunçel, Muzaffer
dc.contributor.authorKanzk, L.
dc.date.accessioned2019-10-19T14:02:51Z
dc.date.available2019-10-19T14:02:51Z
dc.date.issued2001
dc.identifier.issn0014-2999
dc.identifier.urihttps://dx.doi.org/10.1016/S0014-2999(01)00843-3
dc.identifier.urihttps://hdl.handle.net/11421/12413
dc.descriptionPubMed: 11275005en_US
dc.description.abstractWe studied the effects of urate, a peroxynitrite scavenger, on ischaemia- and peroxynitrite-induced preconditioning in rat isolated hearts. Isolated hearts perfused with Krebs-Henseleit solution were preconditioned either by 3 min of coronary artery occlusion or by peroxynitrite administration (1 µM) for 3 min, followed by 10 min of reperfusion and 30 min of coronary artery occlusion. Both ischaemia and peroxynitrite produced a marked reduction in arrhythmias. Urate (1 mM) added to the perfusate 10 min prior to ischaemic preconditioning or peroxynitrite infusion and maintained until coronary artery occlusion, markedly reversed the beneficial effects in the ischaemic and peroxynitrite-treated groups. Urate administration in the peroxynitrite-treated group increased the incidence of ventricular tachycardia from 57% (n = 11) to 100% (n = 6) and total ventricular fibrillation from 0% (n = 0) to 44% (n = 4). Similarly, urate augmented the incidence of ventricular tachycardia from 47% (n = 8) to 85% (n = 6) in the ischaemic preconditioning group. On its own, urate did not affect the severity of cardiac arrhythmias. Peroxynitrite infusion caused a marked increase in the effluent nitrate levels, from 0.05 ± 0.1 µM (n = 5) to 0.4 ± 0.2 µM (n = 6), and urate significantly decreased these levels to 0.08 ± 0.03 µM (n = 9). These results suggest that peroxynitrite at low concentrations contributes to the beneficial effects of preconditioning on ischaemia-induced arrhythmias in rat isolated hearts.en_US
dc.description.sponsorshipSBE-11/99-05en_US
dc.description.sponsorshipThis study was supported by a research grant from Gazi University (SBE-11/99-05).en_US
dc.language.isoengen_US
dc.relation.isversionof10.1016/S0014-2999(01)00843-3en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIschaemia-Induced Arrhythmiaen_US
dc.subjectNitrate Levelen_US
dc.subjectPeroxynitriteen_US
dc.subjectPreconditioningen_US
dc.subjectUrateen_US
dc.titleContribution of peroxynitrite to the beneficial effects of preconditioning on ischaemia-induced arrhythmias in rat isolated heartsen_US
dc.typearticleen_US
dc.relation.journalEuropean Journal of Pharmacologyen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Analitik Kimya Anabilim Dalıen_US
dc.identifier.volume415en_US
dc.identifier.issue2.Maren_US
dc.identifier.startpage239en_US
dc.identifier.endpage246en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorAk, Dilek
dc.contributor.institutionauthorTunçel, Muzaffer


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