Activity Guided Fractionation of Arum italicum Miller Tubers and the LC/MS-MS Profiles

Abstract

In this study, the polar extract of Arum italicum Miller tubers was fractionated by ` biological activity guided fractionation' method. MCF-7 (human breast adenocarcinoma) and A549 (non-small lung cancer) cancer cell lines were used to screen cytotoxic activity. Possible antiproliferative and apoptotic effects of each subfraction were evaluated by in vitro. As a result, E2 were cytotoxic against A549 cell lines while E3 and E6 showed good cytotoxic activities against both cancer cell lines. These three subfractions showed apoptotic and antiproliferative effects comparable with positive control. The chemical composition of each subfraction was determined by LC/MS-MS. Hydroxycinnamic acid derivatives, lignans and glycosides, hydroxycinnamic acidspermidine conjugates and oxylipins identified in subfractions were major secondary metabolites.