Phytochemical profiling and evaluation of marrubium sivasense aytaç, akgül & ekici for antioxidant activity and inhibition effects on ?-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes

Abstract

In the present study, the extract of an endemic plant ourkey Marrubium sivasense Aytaç, Akgül & Ekici was investigated for phytochemical profile, antioxidant properties and inhibition of several enzymes. The chemical composition of the extract was investigated with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified as the main compounds. The free radical scavenging activity of the extract was determined against DPPH• radicals (IC50 0.34 mg/mL) and ABTS•+ cation radicals (TEAC 2.3 mM). In the xanthine-xanthine oxidase (XO) system and in ß-carotene bleaching assay the extract demonstrated moderate activity (Inh% 16.54 and Inh% 43±1.26, respectively). The extract demonstrated hypoglycemic activity (Inh% 49.67±1.09) via inhibition of porcine pancreatic ?-amylase. The antiinflammatory effect tested via inhibition of 5-LOX was found as Inh% 18.71±0.74. However, no antityrosinase activity was found