Antioxidant, Antimicrobial and Anticholinesterase Activities of Ferulago pauciradiata Boiss. & Heldr. Growing in Turkey

Abstract

Ferulago species have been utilised since ancient times as antihelmentic, peptic, sedative and aphrodisiac, and as the seasoning in view of their special odors. The study reports the antioxidant, antimicrobial and anticholinesterase activities of essential oil of fruit, extracts, fractions from roots and aerial parts and isolated compounds from dichloromethane fraction of root [prantschimgin (1), felamidin (2)] from F. pauciradiata. GC and GC/MS were used for analysis of essential oil. Phenolic contents and antioxidant activities of specimens were performed via Folin-Ciocalteu’s phenol indicator, DPPH and TBA methods. Anticholinesterase activity was carried out via Ellman’s method. They were also assessed for antimicrobial activity by macrobroth-dilution and microbroth-dilution procedures. Trans-chrysanthenyl acetate, ?-pinene, 2,3,6-trimethyl benzaldehyde, sabinene and myrcene were gained as primary components of essential oil. Highest total phenolic content was found in EtOAc fraction of root. Highest DPPH activity was observed in essential oil and EtOAc fraction of root (4.59, 6.56 µg/mL, respectively). Among the samples essential oil showed best activity against all microorganism with between 0.097 - 12.5 µg/mL MIC values. Essential oil demonstrated strong inhibition against AChE and BuChE (IC 50 = 7.987, 0.567 µl/ml, respectively). It displayed inhibitory activities on AChE and BuChE, moreover considerable antioxidant and antimicrobial activities