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dc.contributor.authorDikmen, Miriş
dc.contributor.authorCantürk, Zerrin
dc.contributor.authorArtagan, Oge
dc.contributor.authorÖztürk, Nilgün
dc.date.accessioned2019-10-19T14:16:37Z
dc.date.available2019-10-19T14:16:37Z
dc.date.issued2017
dc.identifier.issn1811-7775
dc.identifier.issn1812-5700
dc.identifier.urihttps://dx.doi.org/10.3923/ijp.2017.227.236
dc.identifier.urihttps://hdl.handle.net/11421/13198
dc.descriptionWOS: 000398158600001en_US
dc.description.abstractObjective: This study aimed to investigate the antioxidant and anticancer effects of the secondary metabolites of halotolerant Aspergillus terreus-2 (hAt-2) on colon adenocarcinoma (Caco-2) cells. The hAt-2 was isolated from Lake Tuz in Turkey. Materials and Methods: Ethyl acetate extract was prepared from the isolate of hAt-2 culture medium. The free radical scavenging activity and total phenolic content were evaluated using 2,2-diphenyl-l-picrylhydrazyl and Folin-Ciocalteu assays. The phenolic acids of the extract were evaluated using the high-performance liquid chromatography method. The cytotoxic effect of the extract was evaluated using 4-[3( 4-iodo-phenyl)-2-(4-nitrophenyl)-2H-5 tetrazolio]-1,3-benzene disulfonate assay. Cell proliferation was evaluated using the real-time cell analysis system (RTCA-DP). Results: The major phenolic acid in the hAt-2 extract was protocatechuic acid. The hAt-2 extract exhibited significant antiproliferative and apoptotic effects on Caco-2 cells in a time and concentration-dependent manner. The IC50 value of the extract was detected as 1859 mu g mL(-1) at 72 h using the RTCA-DP system. Conclusion: The present study suggested that the hAt-2 extract has antioxidant, antiproliferative and apoptotic properties to act as an anti-cancer agent on Caco-2 cells.en_US
dc.description.sponsorshipAnadolu University Scientific Research Project Unit [1003S117]en_US
dc.description.sponsorshipThis study was supported by Anadolu University Scientific Research Project Unit (No. of project: 1003S117). The flow cytometric analysis was performed at the Medicinal Plants, Drugs and Scientific Research Center of Anadolu University.en_US
dc.language.isoengen_US
dc.publisherAsian Network Scientific Information-Ansineten_US
dc.relation.isversionof10.3923/ijp.2017.227.236en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticanceren_US
dc.subjectApoptoticen_US
dc.subjectAspergillus Terreusen_US
dc.subjectCaco-2en_US
dc.subjectSecondary Metaboliteen_US
dc.titleAntioxidant, Antiproliferative and Apoptotic Effects of Secondary Metabolites of Halotolerant Aspergillus terreus on Colon Adenocarcinoma Caco-2 Cellsen_US
dc.typearticleen_US
dc.relation.journalInternational Journal of Pharmacologyen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalıen_US
dc.identifier.volume13en_US
dc.identifier.issue3en_US
dc.identifier.startpage227en_US
dc.identifier.endpage236en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorDikmen, Miriş
dc.contributor.institutionauthorCantürk, Zerrin
dc.contributor.institutionauthorÖztürk, Nilgün


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