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dc.contributor.authorGüven, Umay Merve
dc.contributor.authorBerkman, Murat Sami
dc.contributor.authorYazan, Yasemin
dc.date.accessioned2019-10-19T16:02:45Z
dc.date.available2019-10-19T16:02:45Z
dc.date.issued2019
dc.identifier.issn1307-2080
dc.identifier.urihttps://dx.doi.org/10.23893/1307-2080.APS.05701
dc.identifier.urihttps://hdl.handle.net/11421/13908
dc.description.abstractA new, simple, rapid, precise, accurate and specific stability indicating reverse phase UPLC method developed for the determination of encapsulated olopatadine hydrochloride (OLO) in the polymeric nanoparticle formulations. Studies were carried out on a 2.1x50 mm, 1.8 µm Zorbax Eclipse Plus C18 column with an optimized mobile phase of methanol, water and sodium acetate buffer solution (40:50:10, v/v/v) at a flow rate of 0.5 mL/min. OLO was detected and quantitated using a photodiode array detector at a wavelength of 246 nm and the column temperature was adjusted to 40 °C. The proposed method validation was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness according to the ICH harmonised tripartite guideline “validation of analytical procedures Q2(R1)”. Analytical curve was linear over the concentration range of 5-50 µg/mL. All the validation parameters were within the acceptance range. LOD and LOQ for OLO were 0.7652 and 2.3188 µg/mL, respectively. The developed method fulfilled the requirements for reliability and feasibility for the quantitative analysis of OLO in polymeric nanoparticlesen_US
dc.description.sponsorship1406S312en_US
dc.description.sponsorshipThis research was supported by Anadolu University Scientific Research Project Committee (No: 1406S312).en_US
dc.language.isoengen_US
dc.publisherİstanbul Medipol Universityen_US
dc.relation.isversionof10.23893/1307-2080.APS.05701en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEudragiten_US
dc.subjectNanoparticlesen_US
dc.subjectOlopatadine Hydroclorideen_US
dc.subjectUplcen_US
dc.subjectValidationen_US
dc.titleDevelopment and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticlesen_US
dc.typearticleen_US
dc.relation.journalActa Pharmaceutica Scienciaen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Farmasötik Teknoloji Anabilim Dalıen_US
dc.identifier.volume57en_US
dc.identifier.issue1en_US
dc.identifier.startpage7en_US
dc.identifier.endpage18en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US]
dc.contributor.institutionauthorBerkman, Murat Sami
dc.contributor.institutionauthorYazan, Yasemin


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