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dc.contributor.authorSarıözlü, Nalan Yılmaz
dc.contributor.authorÇakır, Erdoğan
dc.contributor.authorKıvanç, Merih
dc.contributor.authorTunçel, Muzaffer
dc.date.accessioned2019-10-20T08:00:36Z
dc.date.available2019-10-20T08:00:36Z
dc.date.issued2011
dc.identifier.issn1996-0808
dc.identifier.urihttps://hdl.handle.net/11421/16126
dc.descriptionWOS: 000293693100012en_US
dc.description.abstractA new high performance liquid chromatography (HPLC) method was developed based on an indirect determination of tannase activity through gallic acid measurement using paracetamol as an internal standard. The separation was performed on a C(18) column using a mobile phase consisting of aqueous formic acid solution (1%) and methanol (85:15; v/v) pumped at 1 ml min(-1). Samples (10 mu l) were injected and signals were detected at 254 nm. Repeatability was 0.81 and 1.28 for peak normalization values of gallic acid (4.16 x 10(-5) M) and internal standard (2.02 x 10(-5) M). A good linearity was shown in the range of 1.04 x 10(-5) to 8.32 x 10(-5) M. Limit of detection (LOD) and limit of quantification (LOQ) values were calculated to be 2.2 x 10(-6) M and 6.6 x 10(-6) M, respectively. The applicability of the method was tested using Penicillium spinulosum which produces tannase. The method was highly sensitive, accurate, reliable, and repeatable and at the same time it is applicable to both pure and crude enzyme.en_US
dc.description.sponsorshipAnadolu University [011024]en_US
dc.description.sponsorshipThe authors gratefully acknowledge for their financial support of the research project (No: 011024) by the Anadolu University Council of Research Project Fund.en_US
dc.language.isoengen_US
dc.publisherAcademic Journalsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTannase Activityen_US
dc.subjectGallic Aciden_US
dc.subjectTannic Aciden_US
dc.subjectHigh Performance Liquid Chromatographyen_US
dc.subjectEnzyme Activityen_US
dc.subjectValidationen_US
dc.titleA method for the determination of tannase activity based on gallic acid measurement by high performance liquid chromatography (HPLC)en_US
dc.typearticleen_US
dc.relation.journalAfrican Journal of Microbiology Researchen_US
dc.contributor.departmentAnadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.identifier.volume5en_US
dc.identifier.issue2en_US
dc.identifier.startpage158en_US
dc.identifier.endpage163en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorKıvanç, Merih
dc.contributor.institutionauthorTunçel, Muzaffer


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