Multilocus enzyme electrophoresis analysis of Pseudomonas syringae pv. phaseolicola

Abstract

A multilocus enzyme electrophoresis technique was developed to detect variation in seven enzyme loci among isolates of Pseudomonas syringae pv. phaseolicola, representing three races from different geographical locations, the causal agent of the halo blight disease of beans. Cellulose acetate gel electrophoresis of seven enzymes revealed 19 electrotypes (ET) among 21 Pseudomonas syringae pv. phaseolicola isolates. One of the pathovar syringae and one of the pathovar tomato isolates were represented by two different ET. The population of Turkish isolates and three races of the pathovar phaseolicola appeared to be genetically diverse.