Antimicrobial activity of centaura derderiifolia, stachys aleurites and anthemis aciphylla

Abstract

The antimicrobial activity of the ethanol, aseton, ethyl asetate and chloroform extracts from Centaurea derderiifolia Wagenitz (Asteraceae), Stachys aleurites Boiss. (Lamiaceae) and Anthemis aciphylla Boiss. var.aciphylla (Asteraceae) were investigated by agar-well diffusion assay. These extracts were tested in vitro against 26 bacterial and 3 yeast species. Etyhl acetate and acetone extracts of C. derderiifolia, S. aleurites and A. a. var. aciphylla showed antimicrobial activity in varying degrees on all the microorganisms tested. However, chloroform and ethanol extracts of all the plants were not active on some microorganisms tested. Ethyl asetate extracts of the samples exhibited stronger and broader spectrum of antimicrobial activity as compared to ethanol, aseton and chloroform extracts. Therefore, minimal inhibitory concentration (MIC) of only ethyl acetate extracts of the samples was determined for seven bacteria chosen from agar-well diffusion assay. MIC values of the extracts were between 250-125 ?g/ml. In conclusion, ethyl asetate extract of C. derderiifolia was the most active against Aeromonas hydrophila both in agar-well diffusion and MIC assays.