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dc.contributor.authorArmutcu, Canan
dc.contributor.authorBereli, Nilay
dc.contributor.authorBayram, Engin
dc.contributor.authorUzun, Lokman
dc.contributor.authorSay, Rıdvan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-20T09:13:50Z
dc.date.available2019-10-20T09:13:50Z
dc.date.issued2014
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfb.2013.08.008
dc.identifier.urihttps://hdl.handle.net/11421/17072
dc.descriptionWOS: 000331596100009en_US
dc.descriptionPubMed ID: 24161508en_US
dc.description.abstractNovel aspartic acid incorporated monolithic columns were prepared to efficiently affinity purify immunoglobulin G (IgG) from human plasma. The monolithic columns were synthesised in a stainless steel HPLC column (20 cm x 5 mm id) by in situ bulk polymerisation of N-methacryloyl-L-aspartic acid (MAAsp), a polymerisable derivative of L-aspartic acid, and 2-hydroxyethyl methacrylate (HEMA). Monolithic columns [poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) (PHEMAsp)] were characterised by swelling studies, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The monolithic columns were used for IgG adsorption/desorption from aqueous solutions and human plasma. The IgG adsorption depended on the buffer type, and the maximum IgG adsorption from aqueous solution in phosphate buffer was 0.085 mg/g at pH 6.0. The monolithic columns allowed for one-step IgG purification with a negligible capacity decrease after ten adsorption-desorption cyclesen_US
dc.language.isoengen_US
dc.publisherElsevier Science BVen_US
dc.relation.isversionof10.1016/j.colsurfb.2013.08.008en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMonolithic Columnsen_US
dc.subjectIgg Separationen_US
dc.subjectPseudospecific Ligandsen_US
dc.subjectHigh Performance Liquid Chromatographyen_US
dc.titleAspartic acid incorporated monolithic columns for affinity glycoprotein purificationen_US
dc.typearticleen_US
dc.relation.journalColloids and Surfaces B-Biointerfacesen_US
dc.contributor.departmentAnadolu Üniversitesi, Fen Fakültesi, Fizik Bölümüen_US
dc.identifier.volume114en_US
dc.identifier.startpage67en_US
dc.identifier.endpage74en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorSay, Rıdvan


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