Use of molecular imprinted nanoparticles as biorecognition element on surface plasmon resonance sensor

Abstract

In this study, we have prepared lysozyme imprinted poly(ethylene glycol dimethacrylate-N-methacryloyl-L-histidine methylester) (PEDMAH) nanoparticles and then attached on the surface of surface plasmon resonance (SPR) sensor. Lysozyme imprinted SPR nanosensor was characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and ellipsometry. The nanosensor has an ability to detect lysozyme molecules from both aqueous and natural complex source, chicken egg white, even if it has lysozyme concentration as low as 32.2 nM. Association kinetics analysis, Scatchard, Langmuir, Freundlich, Langmuir-Freundlich isotherms were applied to data. The calculated detection limit, association and dissociation constants are 84 pM, 108.71 nM(-1) and 9.20 pM, respectively. The non-imprinted nanosensors were also prepared to evaluate the selectivity of the imprinted nanosensor. Finally, the nanosensor is used for five adsorption-desorption-regeneration cycle and it gives reproducible response