Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith as a stationary phase in dye-affinity chromatography

Abstract

Porous monolith was obtained by the bulk polymerization of ethylene dimethacrylate (EDMA) and glycidyl methacrylate (GMA) conducted in a glass tube. Poly(EDMA-GMA) monolith had a specific surface area of 98.7 m(2)/g. Poly(EDMA-GMA) monolith was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. Poly(EDMA-GMA) monolith with a swelling ratio of 48%, and containing 45.7 mumol Cibacron Blue F3GA/g, were used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.8 mg/g). The maximum amount of HSA adsorption from aqueous solution in phosphate buffer was 22 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 53.2 mg/g) with a purity of 92%. Desorption of HSA from Cibacron Blue F3GA-attached poly(EDMA-GMA) monolith was obtained using 0.1 M Tris/HCl buffer containing 0.5 M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(EDMA-GMA) monolith without significant loss in the adsorption capacity.