Effects of the N-Ras oncogene on the expression of other cellular genes in CO25 myoblast cells

Abstract

The effects of the N-ras oncogens on the transcription levels of c-myc, p53, c-K-ras and beta-actin genes have been investigated by Northern blotting in a cell line which normally differentiates into myotubes. Treatment of cells with dexamethasone activates N-ras gene, then cells proliferate and form foci. The transcription level of c-myc gene was generally high during transformation whereas c-K-ras mRNAs levels were high during differentiation. Following induction of the N-ras oncogene, the level of c-myc mRNA first decreased in two days and then increased in three and four days. During differentiation, c-K-ras mRNA decreased in three days and increased following day. The level of beta-actin mRNA was slightly lower during transformation, and there were no changes in the mRNA levels of p53 and c-K-ras. An increase in the level of c-myc mRNA in four days of differentiation when myo-tubes form, shows that expression of c-myc may not have an effect after this critical period of differentiation.

The effects of the N-ras oncogens on the transcription levels of c-myc, p53, c-K-ras and beta-actin genes have been investigated by Northern blotting in a cell line which normally differentiates into myotubes. Treatment of cells with dexamethasone activates N-ras gene, then cells proliferate and form foci. The transcription level of c-myc gene was generally high during transformation whereas c-K-ras mRNAs levels were high during differentiation. Following induction of the N-ras oncogene, the level of c-myc mRNA first decreased in two days and then increased in three and four days. During differentiation, c-K-ras mRNA decreased in three days and increased following day. The level of beta-actin mRNA was slightly lower during transformation, and there were no changes in the mRNA levels of p53 and c-K-ras. An increase in the level of c-myc mRNA in four days of differentiation when myo-tubes form, shows that expression of c-myc may not have an effect after this critical period of differentiation.