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dc.contributor.authorCaglayan, Cuneyt
dc.contributor.authorTaslimi, Parham
dc.contributor.authorTurk, Cebrahil
dc.contributor.authorGulcin, Ilhami
dc.contributor.authorKandemir, Fatih Mehmet
dc.contributor.authorDemir, Yeliz
dc.contributor.authorBeydemir, Sukru
dc.date.accessioned2020-07-09T20:58:45Z
dc.date.available2020-07-09T20:58:45Z
dc.date.issued2020
dc.identifier.issn0944-1344
dc.identifier.issn1614-7499
dc.identifier.urihttps://doi.org/10.1007/s11356-020-07611-z
dc.identifier.urihttps://hdl.handle.net/11421/24010
dc.descriptionWOS: 000507371600006en_US
dc.descriptionPubMed: 31942715en_US
dc.description.abstractThe gill tissue is the main site of metabolic enzymes or compensation, with the kidney tissue playing a supporting role. At the gill tissue, carbonic anhydrase enzymes (CAs) catalyze the hydration of CO2 to HCO3- and H+ for production to the H2O. in this work, the CA enzyme was purified from horse mackerel (Trachurus trachurus) gill with a specific activity of 21,381.42 EU/mg, purification fold of 150.61, total activity of 2347.68 EU/mL, and a yield of 16.13% using sepharose 4B-L-tyrosine-sulfanilamide affinity gel chromatography. For recording the enzyme purity, gel electrophoresis was performed, and single band was seen. the molecular weight of this enzyme was found approximately 35 kDa. Also, the inhibitory effects of different pesticides such as thiram, clofentezine, propineb, deltamethrin, azoxystrobin, and thiophanate and heavy metal ions such as Fe2+, Cu2+, Co2+, Pb2+ Hg2+, and As3+ on horse mackerel gill tissue CA enzyme activities were investigated. Our results indicated that these pesticides and metal ions showed inhibitory effects at low nanomolar and millimolar concentrations for fish gill CA enzymes, respectively.en_US
dc.language.isoengen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.isversionof10.1007/s11356-020-07611-zen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectHorse mackerelen_US
dc.subjectTrachurus trachurusen_US
dc.subjectGillen_US
dc.subjectHeavy metalen_US
dc.subjectPesticidesen_US
dc.titleInhibition effects of some pesticides and heavy metals on carbonic anhydrase enzyme activity purified from horse mackerel (Trachurus trachurus) gill tissuesen_US
dc.typearticleen_US
dc.relation.journalEnvironmental Science and Pollution Researchen_US
dc.contributor.departmentAnadolu Üniversitesien_US
dc.identifier.volume27en_US
dc.identifier.issue10en_US
dc.identifier.startpage10607en_US
dc.identifier.endpage10616en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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