Effects of Different Derivatives of Eudragit Polymer on Entrapment Efficiency, In Vitro Dissolution, Release Kinetics and Cell Viability Results on Extended Release Flurbiprofen Loaded Nanomedicines

Abstract

The purpose of the present investigation was to design and compare the release characteristics of sustained-release formulations of Flurbiprofen (FLB) loaded nanoparticles (NPs) by using spray-dryer technique using Eudragit RLPO (RLPO), Eudragit RSPO (RSPO) and RLPO:RSPO 1:1 ratio. NPs Formulation were prepared by spray-dryer. Structures of nanoparticles were characterized by entrapment efficiency (EE%), dissolution study and release kinetic study with the DDSolver software program, particle size (PS), zeta potential, morphology, DSC, XRD, FTIR and H-1-NMR analyses. Cytotoxicity studies were performed on the NIH/3T3 mouse embryonic fibroblast cells. FLB-loaded NPs demonstrated nanostructural character while in vitro release study showed extended release of FLB-incorporated. The PS of the prepared FLB-NPs was affected by the polymer type and was in the range of 532 and 565 nm. Entrapment efficiency (EE %) varied from 70 to 76%, depending upon the polymer difference to drug ratio. According to the DDSolver criterion used to evaluate release kinetics, Korsmeyer-Peppas model were determined to be the most appropriate kinetic models for NPs. The results obtained in cell viability study indicate a dose and time dependent decrease in viability of NIH/3T3 for FLB and FLB-NPs. As a conclusion of this study; the effect of the polymer type on the PS, EE% and release properties of NPs has been examined and discussed in detail. According to results FLB-NPs seem to be a promising extended release drug delivery system for oral administration.