Investigation of Genotoxic Effects of Some Ruthenium Complexes According to Cis-platinum

Abstract

The aim of our study was, to synthesize and evaluate the mutagenicity of Ruthenium complexes, on Salmonella strains of TA100 and TA98 by Ames Test. [Ru(L)(2)(B)Pt] Cl-2 complexes were synthesized using 1, 10-phenanthroline-5,6-dione (dp) (L1), imidazo [4,5-f] [1,10] phenanthroline (ip) (L2) and 2-phenylimidazo [4,5-f] [1, 10] phenanthroline (pip) (L3) as ligands and bis-1,4-di [([1, 10] phenantrolin-5-il) amino]-2-buten (B) as a bridge molecule. The characterization of the intermediate and final compounds arising from this work will be carried out by means of a variety of spectroscopic methods, which include H-1 NMR, IR, MS and elemental analysis. The mutagenicity of [Ru (L)(2) (B) Pt] Cl-2 complexes, was investigated by the Ames Salmonella assay with strains TA 98 carrying a frameshift mutation and TA 100 base-pair substitution mutation were used in plate incorporation method in the absence of metabolic activation. RuL2BPt complex on TA98 Salmonella strain was found mutagenically effective at the dose of 100 mu g/plt. RuL3BPt complex gave mutagenic response on TA98 strain at doses of 25, 50, 100 mu g/plt, maximum mutagenic response was obtained at 25 mu g/plt dose. There was no significant increase of revertants due to the treatment with the doses of 5, 10, 25, 50 mu g/plt cis-platin on TA98. 100 mu g/plt dose showed mutagenic effect on TA98. On TA 100 strain dose related increase in the number of revertant colonies was observed but the number of revertants were not double the number of spontaneous colonies up to 100 mu g/plt dose. Only 100 mu g/pit dose showed mutagenic effect. The test compounds were directly effective, especially in the TA 98 Strain of Salmonella. Ruthenium derivatives was not mutagenic on the Salmonella strain of TA100.