Dexketoprofen trometamol loaded solid lipid nanoparticles(SLNs): Formulation, in vitroand in vivoevaluation

Abstract

Development, in vitroand in vivoevaluation of dexketoprofen trometamol (DT)-loaded nanosizeddrugdelivery system was aimed in this study. DT-loaded solid lipid nanoparticles (SLNs) were prepared using probe sonication. DT release from SLNs prepared and their kinetics were investigated. Structures of SLNs were elucidated by particle size and zeta potential measurements, shape and surface imaging, thermal analysis,X-ray diffraction, FT-IR and 1H-NMR determinations. DT-loaded particles demonstrated characteristic plaque shapes while in vitrorelease studies showed extended release of DT. Korsmeyer-Peppas kinetic model was found to fit the best using DDSolversoftware program. Stability, cytotoxicity and in vivoanimal experiments were further performed on DT-loaded SLN showing also prolonged analgesic activity in mice. Depending on the in vitroand in vivotest results, formulation developed in this study seems to prolong DT release and is promising for extending analgesic activity.