Bildiri Koleksiyonuhttps://hdl.handle.net/11421/121872024-03-28T23:42:55Z2024-03-28T23:42:55ZInvestigation of Galatella villosa and G. tatarica for antioxidant, ?-amylase, tyrosinase, lipoxygenase and xanthine oxidase inhibitory activitiesÖzek, GülmiraIshmuratova, MargaritaYur, S.Göğer, FatihHaliloğlu, YeşimBaşer, K. Hüsnü CanÖzek, Temelhttps://hdl.handle.net/11421/130942020-08-16T08:22:13Z2017-01-01T00:00:00ZInvestigation of Galatella villosa and G. tatarica for antioxidant, ?-amylase, tyrosinase, lipoxygenase and xanthine oxidase inhibitory activities
Özek, Gülmira; Ishmuratova, Margarita; Yur, S.; Göğer, Fatih; Haliloğlu, Yeşim; Başer, K. Hüsnü Can; Özek, Temel
In the present work two Galatella species, Galatella villosa Rchb.f. and Galatella tatarica (Less.) Novopokr collected from Kazakhstan were subjected to hydrodistillation to yield essential oils (EOs), and to maceration in methanol to yield extracts (MEs). The EOs and MEs were evaluated for antioxidant and in vitro inhibitory activities against ?-amylase, tyrosinase, lipoxygenase and xanthine oxidase enzymes. The EOs of G. villosa and G. tatarica were investigated for chemical composition using GC-FID and GC/MS techniques. The composition of MEs was investigated with LC-MS/MS technique. Monoterpenes, ß- pinene (23.6 %) and ?-pinene (14.4 %) were the main constituents of G. tatarica essential oil, while the oil of G. villosa was characterized not only with monoterpene ?-pinene (9.0 %), but also fatty acid, hexadecanoic acid (10.2%). 3-, 5-, 1,5- and 3,4-caffeoylquinic acids, 3-, 4- and 5-feruloylquinic acids, quercetin and its different glycosides were detected in MEs according to mass spectral data. G. tatarica (IC 50 =74.3 µg/mL) and G. villosa (IC 50 =78.5 µg/mL) extracts showed significant antioxidant activity. In the ß-carotene bleaching test, the extracts demonstrated noteworthy activity (%Inh 44.46 and 41.14). The extracts scavenged ABTS radical as 1.85 mM and 1.27 mM of Trolox. In a concentration of 0.5 mg/mL, the oils showed 89% and 85% inhibition of ?-amylase, while the extracts showed 86% and 78% inhibition at 2 mg/mL concentration, respectively. The EOs demonstrated noteworthy potent LOX enzyme inhibition ranging between 45% and 57%. In xanthine-xanthine oxidase model system, the extracts demonstrated the highest inhibitory potency ranging between 43% and 39%. The present work is the first contribution to the chemistry and biological activities of G. tatarica and G. villosa.
2017-01-01T00:00:00ZAntioxidant activities of Molokhia (Corchorus olitorius L.) extractsÖztürk, NilgünSavaroğlu, F.https://hdl.handle.net/11421/130842020-02-19T20:19:30Z2011-01-01T00:00:00ZAntioxidant activities of Molokhia (Corchorus olitorius L.) extracts
Öztürk, Nilgün; Savaroğlu, F.
Corchorus olitorius L. (Molokhia) (Tiliaceae), collected from the Doganci, Güzelyurt, Northern Cyprus, infusion and extracts with different polarities have been studied for their polyphenolic contents and antioxidant activities. Total phenolic content was determined spectrometrically according to the Folin-Ciocalteu method and calculated as gallic acid equivalents (GAE). Antioxidant activitiy was studied in an aqueous emulsion system of ß-carotene and linoleic acid by measuring the absorbance of the samples. The free radical-scavenging properties were also evaluated against 2,2-diphenyl-1-picryhydrazyl radical (DPPH•). Results were compared those of an synthetic antioxidant, BHT. Antioxidant effects were correlated with the total amount of phenolic compounds contained in the extracts. In all these cases higher antioxidant activity was seen in the samples with higher phenolic content
International Conference on Environment: Survival and Sustainability -- 19 February 2007 through 24 February 2007 -- Nicosia -- 102775
2011-01-01T00:00:00ZDetermination of quality specifications of origanum vulgare subsp. hirtum populations growing in marmara region of turkeyTınmaz, A. B.Başer, K. Hüsnü CanKarık, Ü.Kürkçüoğlu, MineÖztürk, Munirhttps://hdl.handle.net/11421/130542020-08-16T08:22:12Z2009-01-01T00:00:00ZDetermination of quality specifications of origanum vulgare subsp. hirtum populations growing in marmara region of turkey
Tınmaz, A. B.; Başer, K. Hüsnü Can; Karık, Ü.; Kürkçüoğlu, Mine; Öztürk, Munir
Herbal parts and seeds of Origanum vulgare subsp. hirtum samples from populations growing in the Marmara region of Turkey have been collected. They were raised at the demonstration garden of Atatürk Central Horticultural Research Institute. Both wild crafted and raised plants herb yield, essential oil yield and composition were determined in order to find superior populations. Superior population of 61 populations planted to irrigated environment of culture and their performance had been observed according to harvest time (before inflorescence, beginning inflorescence, whole inflorescence), characteristics of yield and quality. The experiment was designed as randomized block with four replications. According characters which had been measured different harvest time had been important on yield and quality of Istanbul Oregano.
2009-01-01T00:00:00ZInvestigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) assayDemirci, FatihPaper, DHFranz, GerhardBaşer, K. Hüsnü Canhttps://hdl.handle.net/11421/130272020-08-16T08:22:11Z2004-01-01T00:00:00ZInvestigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) assay
Demirci, Fatih; Paper, DH; Franz, Gerhard; Başer, K. Hüsnü Can
The in vivo test on the chorioallantoic membrane of the fertilized hen's egg (CAM assay) is a current method to determine antiangiogenic, antiinflammatory activity and toxic effects of individual compounds or complex plant extracts. The method is used for testing natural compounds in small amounts for revealing various modes of action and the complex mechanisms related to angiogenesis and inflammation. Furthermore, possible side effects Such as membrane irritation, toxic, and anticoagulant properties of the investigated material in question can be detected. For the evaluation, the essential oil obtained by hydrodistillation of the aerial parts of Origanum onites L., a common spice and medicinal plant, was tested for its effect in the chorioallantoic membrane (CAM) assay. The essential oil composition was revealed by means of gas chromatography-mass spectrometry (GC-MS). Eighty three components were identified, representing 99.1% of the total oil. Carvacrol, thymol, p-cymene, and gamma-terpinene were found as major components and were also individually tested in the CAM assay. Along with the monoterpenes carvacrol and thymol, their methyl ether derivatives were also examined for comparison of their physiological action. Neither the essential oil nor its components showed any pronounced antiinflammatory or antiangiogenic property in the CAM assay, at 10-250 mug/pellet. However, the irritant effect of the essential oil was linked to thymol in a dose-response fashion, up to 10 mug/pellet, where it was still showing irritation.
3rd World Congress on Medicinal and Aromatic Plants for Human Welfare (WOCMAP-III) -- FEB 03-07, 2003 -- CHIANG MAI, THAILAND; WOS: 000188366300017; PubMed ID: 14733504
2004-01-01T00:00:00Z