Makale Koleksiyonu
https://hdl.handle.net/11421/12183
2024-03-29T01:03:53ZPhylogenetic relationships between malcolmia, strigosella, zuvanda, and some closely related genera (brassicaceae) from turkey revealed by inter-simple sequence repeat amplification
https://hdl.handle.net/11421/12546
Phylogenetic relationships between malcolmia, strigosella, zuvanda, and some closely related genera (brassicaceae) from turkey revealed by inter-simple sequence repeat amplification
Doğan, Bekir; Ünal, Murat; Özgökçe, Fevzi; Kaya, Ayla
The genus Malcolmia W.T.Aiton is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR primers, and the phylogenetic relationship among the taxa was revealed through a dendrogram produced as the outcome of the NTSYSpc 2.1 software. The infrageneric and intergeneric phylogenetic relationships among Malcolmia and other related genera are determined. A very close relationship was determined between Malcolmia chia and M. flexuosa. Strigosella and Zuvanda species were determined to be phylogenetically different from these 2 species. The phylogenetic relationships among the Malcolmia, Strigosella, Zuvanda, Leptaleum DC., Neotorularia Hedge & J.Léonard, and Sisymbrium L. taxa were investigated. The phylogenetic separation of Malcolmia, Strigosella, Zuvanda Leptaleum, Neotorularia, and Sisymbrium genera and their specimens constituted separate clades on the dendrogram.; The genus Malcolmia W.T.Aiton is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR primers, and the phylogenetic relationship among the taxa was revealed through a dendrogram produced as the outcome of the NTSYSpc 2.1 software. The infrageneric and intergeneric phylogenetic relationships among Malcolmia and other related genera are determined. A very close relationship was determined between Malcolmia chia and M. flexuosa. Strigosella and Zuvanda species were determined to be phylogenetically different from these 2 species. The phylogenetic relationships among the Malcolmia, Strigosella, Zuvanda, Leptaleum DC., Neotorularia Hedge & J.Léonard, and Sisymbrium L. taxa were investigated. The phylogenetic separation of Malcolmia, Strigosella, Zuvanda Leptaleum, Neotorularia, and Sisymbrium genera and their specimens constituted separate clades on the dendrogram.
2011-01-01T00:00:00ZIn vitro alpha-Glucosidase and alpha-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts
https://hdl.handle.net/11421/12547
In vitro alpha-Glucosidase and alpha-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts
Saltan, Fatma Zerrin; Okutucu, Burcu; Canbay, Hale Seçilmis; Özel, Dilara
This study was performed to demonstrate alpha-glucosidase and alpha-amylase enzyme inhibitor activities using the methanolic extracts of Elaeagnus angustifolia leaves. Methanolic extracts were prepared by two different extraction methods [accelerated solvent extractor (ASE) and Soxhlet apparatus]. The analytical procedure involved the application of liquid chromatography. Our results showed that the ASE extracts inhibited a-amylase (10-100 mu g/ml) and alpha-glucosidase (10-50 mu g/ml) dose-dependently exhibitedrp inhibitory activities with the extracts [alpha-amylase (40%) and alpha-glucosidase (56%)]. RP-HPLC analyses of the methanolic extracts were detected with vanillic acid and 4-hydroxybenzoic acid as the main phenolic acids in all the ASE extracts. Elaeagnus angustifolia leaf extracts may be attributed to the presence of vanillic acid and 4-hydroxybenzoic acid, there by confirming with its traditional use for the management of diabetes mellitus. We implicate that the anti-diabetic activity through in vitro assessments and support to the folkloric use of this plant for controlling type-2 diabetes mellitus in Turkey.
WOS: 000412774100009
2017-01-01T00:00:00ZInvestigation for anti-inflammatory and anti-thrombotic activities of methanol extract of Capparis ovata buds and fruits
https://hdl.handle.net/11421/12545
Investigation for anti-inflammatory and anti-thrombotic activities of methanol extract of Capparis ovata buds and fruits
Bektaş, Nurcan; Arslan, Rana; Göğer, Fatih; Kırımer, Neşe; Öztürk, Yusuf
Ethnopharmacological relevance: Capparis ovata Desf. has wide natural distribution in Turkey and it is consumed in pickled form. Flower buds, root bark, and fruits of the plant are used traditionally due to their analgesic, anti-inflammatory, wound healing, anti-rheumatismal, tonic, and diuretic effects. Aim of the study: The aim of this study was to investigate the possible anti-inflammatory and anti-thrombotic effects of methanol extracts prepared from flower buds (CBE) and fruits (CFE) of C. ovata. Materials and methods: Anti-inflammatory effects of CBE and CFE were assessed using carrageenan-induced and prostaglandin E-2-induced mouse paw edema models. For the anti-thrombotic effect evaluation, carrageenan-induced tail thrombosis model was performed in mice. The extracts were administered intraperitonally (i.p.) at the doses of 100, 200, and 300 mg/kg. The anti-inflammatory effect of Capparis extracts were tested in comparison to 10 mg/kg diclofenac and anti-thrombotic activity to 10 and 100 IU heparin. Results: CBE at the doses of 200, and 300 mg/kg and CFE at the doses of 100, 200, and 300 mg/kg showed significant anti-inflammatory activity and CFE reached therapeutic concentration early than CBE in carrageenan inflammation model. In prostaglandin E-2 inflammation model, CBE and CFE exhibited significant inhibitory effects. The C. ovata extracts did not show remarkable anti-thrombotic effect. Conclusions: Based on the results obtained, it can be concluded that fruits of C. ovata have more potent anti-inflammatory effect than flower buds. It has been suggested that inhibition of cyclooxygenase pathway is one of the mechanisms of the activity. C. ovata may be potentially used as therapeutic agents for inflammatory diseases
WOS: 000305850500007; PubMed ID: 22579676
2012-01-01T00:00:00ZAntinociceptive activity of methanol extract of fruits of Capparis ovata in mice
https://hdl.handle.net/11421/12542
Antinociceptive activity of methanol extract of fruits of Capparis ovata in mice
Arslan, Rana; Bektaş, Nurcan; Öztürk, Yusuf
Ethnopharmacological relevance: Capparis ovata Desf. and Capparis spinosa L have wide natural distribution in Turkey and they are consumed in pickled form. Flower buds, root bark, and fruits of the plant are used in folk medicine due to their analgesic, wound healing, cell regeneration, tonic, and diuretic effects. Aim of the study: In this study, we attempted to identify the possible antinociceptive action of methanol extract prepared from fruits of Capparis ovata. Materials and methods: Using tail immersion, hot plate and writhing tests, the antinociceptive effect of the methanol extract of Capparis ovata (MEC) fruits was assessed after intraperitoneal administration into mice. Morphine sulfate (5 mg/kg; i.p.) and diclofenac (10 mg/kg; i.p.) were used as reference analgesic agents. Naloxone (5 mg/kg: i.p.) was also tested. Results: MEC was studied at the doses of 50, 100, and 200 mg/kg (i.p.) and exhibited significant antinociceptive activities in all tests used. The above-mentioned doses of the extract reduced the writhing responses by 32.21, 55.70, and 68.36%, respectively. MPE% were increased by 7.27, 12.07, 14.60% in the tail immersion, and 7.88, 11.71, 16.73% in the hot plate test at the tested doses, respectively. Naloxone antagonized antinociceptive effect at the doses of 100 and 200 mg/kg whereas partially antagonized the effect of MEC at the dose of 50 mg/kg. Conclusions: Based on the results obtained, it can be concluded that MEC has antinociceptive effects both at the peripheral and central levels
WOS: 000281325500005; PubMed ID: 20595018
2010-01-01T00:00:00Z