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dc.contributor.authorÇalışkan, Figen
dc.contributor.authorSivas, Hülya
dc.contributor.authorŞahin, Yalçın
dc.descriptionWOS: 000270935300006en_US
dc.description.abstractObjectives: Despite the long history on proteolytic activity studies, no study has been reported about the presence of proteases from the Turkish scorpion A. crassicauda venom which is among the most toxic species in the world. The present work is a preliminary study indicating the presence of gelatinolytic proteases in the A. crassicauda crude venom which almost nothing is known about its components. Methods: Protein contents of the venom were analyzed by electrophoresis. Further, the venom fractions were separated by liquid chromatography. Proteolytic activities of crude venom and each fraction were determined by zymogram assay. Results: Two proteins with gelatinolytic activity were detected between 36-45 kDa and 97-116 kDa in the soluble venom at a range of pH 4.0 to 8.0. Proteolytic bands from the fractions obtained chromatographically were detected as three bands between 14.2 and 20 kDa; one band between 20 and 24 kDa; three bands between 24 and 29 kDa; two bands between 97 and 116 kDa at pH 7. Conclusion: Here, first electrophoretic separation of the venom from A. crassicauda where the proteolytic activity against gelatin is reported. These enzymes may prove to be a useful tool for the study of toxin processing and understanding the mechanism of venom activation.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TBAG-106T527]en_US
dc.description.sponsorshipWe would like to thank Dr. Hakan Caliskan and Dr. Umit Sirin from Biology Department of Eskisehir Osmangazi University, who captured scorpions used in this protocol. This research is a part of the doctoral thesis by Figen Caliskan and was partially supported by a grant from the Scientific and Technological Research Council of Turkey (TBAG-106T527).en_US
dc.publisherWalter De Gruyter GMBHen_US
dc.subjectAndroctonus Crassicaudaen_US
dc.subjectTurkish Black Scorpionen_US
dc.titleA Preliminary Study for the Detection of Gelatinolytic Proteases from the Scorpion Androctonus crassicauda (Turkish Black Scorpion) Venomen_US
dc.relation.journalTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.contributor.departmentAnadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorSivas, Hülya

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