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dc.contributor.authorBaşpınar, Mustafa Serhat
dc.contributor.authorÖzyurt, Rumeysa
dc.contributor.authorKuş, Gökhan
dc.contributor.authorKutlay, Özden
dc.contributor.authorÖzkurt, Mete
dc.contributor.authorErkasap, Nilüfer
dc.contributor.authorKabadere, Selda
dc.descriptionWOS: 000407091600003en_US
dc.descriptionPubMed ID: 28766347en_US
dc.description.abstractOBJECTIVE: The aim of this study was to investigate the effects of a novel anti-cancer drug, ceranib-2, which targets the acid ceramidase, in human colon cancer cell line. MATERIALS AND METHODS: The cell lines were treated with 50 mu M of ceranib-2. Relative mRNA expression of TNF-alpha, TNF-R1 and ASAH were assessed by quantitative RT-PCR. RESULTS: Ceranib-2 reduced cell viability in a dose-dependent manner and the apoptotic values of cells following treatment with the dose of 50 mu M were reduced significantly both at 24 h and 48 h compared to the control cells (p < 0.001). TNF-alpha receptor 1 (TNF-R1) mRNA levels were reduced significantly in the cell lines treated with both 25 mu M and 50 mu M of ceranib-2 for 24 h compared to the control cells (p < 0.05), whereas the difference between the treatment and the control cell lines diminished at 48 h. The human acid ceramidase gene (ASAH) mRNA levels were significantly higher in the cell lines treated with 50 mu M of ceranib-2 for 48 h than in the other cell lines (p < 0.001). CONCLUSION: The study shows that ceranib-2 increased apoptosis by inducing ASAH expression and reduced TNF-R1 expression in human colon cancer cell lines in a dose and time-dependent manner (Fig. 3, Ref. 17). Text in PDF
dc.description.sponsorshipEskisehir Osmangazi University Scientific Research Projects Committee [2012/11032]en_US
dc.description.sponsorshipThis study was supported by a grant (project number: 2012/11032) from Eskisehir Osmangazi University Scientific Research Projects Committee.en_US
dc.publisherComenius Universityen_US
dc.subjectColon Canceren_US
dc.titleEffects of ceranib-2 on cell survival and TNF-alpha in colon cancer cell lineen_US
dc.relation.journalBratislava Medical Journal-Bratislavske Lekarske Listyen_US
dc.contributor.departmentAnadolu Üniversitesi, Açıköğretim Fakültesi, Sağlık Yönetimien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorKuş, Gökhan

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