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dc.contributor.authorÖztopçu-Vatan, Pınar
dc.contributor.authorKuş, Gökhan
dc.contributor.authorMan, Emine
dc.contributor.authorKorkut, Melek Gunindi
dc.contributor.authorKabadere, Seida
dc.contributor.authorUyar, Ruhi
dc.date.accessioned2019-10-18T19:26:38Z
dc.date.available2019-10-18T19:26:38Z
dc.date.issued2017
dc.identifier.issn0030-9923
dc.identifier.urihttps://dx.doi.org/10.17582/journal.pjz/2017.49.3.811.817
dc.identifier.urihttps://hdl.handle.net/11421/11552
dc.descriptionWOS: 000409565700007en_US
dc.description.abstractDespite clinical treatments, hepatocellular carcinoma is one of the most common causes of cancer death. We tested whether licofelone, an inhibitor of both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism, changes the survival rate and induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey's multiple comparison test. IC50 values of licofelone were determined as 171 mu M for 24 h and 140 mu M for 48 h applications. Used as a positive control, 250 mu M 5-Fluorouracil decreased the cell survival by only 40 % after 48 h, but licofelone treatment with the same dose and time duration decreased the number of surviving cells by 87 %. Treatments with 150, 200 and 250 mu M licofelone caused early apoptotic cell rates of 4, 15 and 24 % for 24 h and 6, 13 and 24 % for 48 h, respectively. In addition, 150, 200 and 250 mu M of 5-Fluorouracil resulted early apoptotic cell values of 13, 12, and 11 % for 24 h and 18, 22 and 16 % for 48 h. This study revealed that licofelone possesses dose and time dependent anti-proliferative and apoptotic properties on hepatoma cells.en_US
dc.language.isoengen_US
dc.publisherZoological Soc Pakistanen_US
dc.relation.isversionof10.17582/journal.pjz/2017.49.3.811.817en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLicofeloneen_US
dc.subjectAnti-Proliferationen_US
dc.subjectApoptosisen_US
dc.subjectHepatomaen_US
dc.subjectIn Vitroen_US
dc.titleLicofelone Inhibits Proliferation of Rat Hepatoma Cellsen_US
dc.typearticleen_US
dc.relation.journalPakistan Journal of Zoologyen_US
dc.contributor.departmentAnadolu Üniversitesi, Açıköğretim Fakültesi, Sağlık Yönetimien_US
dc.identifier.volume49en_US
dc.identifier.issue3en_US
dc.identifier.startpage811en_US
dc.identifier.endpage817en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorKuş, Gökhan


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