The determination of albendazole by flow injection analysis method using UV-detection and HPLC method in suspensions

Abstract

A simple flow injection analysis (FIA) method of albendazole (ALB) using UV detection and a HPLC method are described in this study. For the FIA method, the best solvent system was found to consist of methanol:HCl:water (49:1:50). The limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 1.2 x 10(-8) and 3.7 x 10(-8) mol.L-1 (n = 8), respectively. For the HPLC method, the mobile phase is a mixture of methanol:acetate buffer (0.05 M, pH:5.8) (70:30 v/v). The retention time obtained was 8.07 min. The limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 1.1 x 10(-8) and 3.8 x 10(-8) mol.L-1 (n = 8), respectively. The proposed methods were applied to the determination of ALB in the suspensions. The results were compared with those obtained from UV spectrophotometry. The validation studies were realised by the related applications and the results were evaluated statistically.