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dc.contributor.authorSeller, Zerrin
dc.contributor.authorHatipoğlu, İbrahim
dc.contributor.authorSivas, Hülya
dc.contributor.authorErgene, Emel
dc.contributor.authorÇiftçi, Gülsen Akalın
dc.date.accessioned2019-10-19T14:03:03Z
dc.date.available2019-10-19T14:03:03Z
dc.date.issued2013
dc.identifier.issn0019-5189
dc.identifier.urihttps://hdl.handle.net/11421/12485
dc.descriptionWOS: 000325511300002en_US
dc.descriptionPubMed ID: 24266102en_US
dc.description.abstractThe aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.en_US
dc.description.sponsorshipAnadolu University of Project Department [030326]en_US
dc.description.sponsorshipThis work was supported by grants from the Anadolu University of Project Department (No. 030326).en_US
dc.language.isoengen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCell Cultureen_US
dc.subjectFibronectinen_US
dc.subjectOsteosarcomaen_US
dc.subjectType Iv Collagenen_US
dc.titleEffects of fibronectin and type IV collagen on osteosarcoma cell apoptosisen_US
dc.typearticleen_US
dc.relation.journalIndian Journal of Experimental Biologyen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalıen_US
dc.identifier.volume51en_US
dc.identifier.issue10en_US
dc.identifier.startpage789en_US
dc.identifier.endpage796en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorSeller, Zerrin
dc.contributor.institutionauthorSivas, Hülya
dc.contributor.institutionauthorÇiftçi, Gülsen Akalın


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