dc.contributor.author | Seller, Zerrin | |
dc.contributor.author | Hatipoğlu, İbrahim | |
dc.contributor.author | Sivas, Hülya | |
dc.contributor.author | Ergene, Emel | |
dc.contributor.author | Çiftçi, Gülsen Akalın | |
dc.date.accessioned | 2019-10-19T14:03:03Z | |
dc.date.available | 2019-10-19T14:03:03Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0019-5189 | |
dc.identifier.uri | https://hdl.handle.net/11421/12485 | |
dc.description | WOS: 000325511300002 | en_US |
dc.description | PubMed ID: 24266102 | en_US |
dc.description.abstract | The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma. | en_US |
dc.description.sponsorship | Anadolu University of Project Department [030326] | en_US |
dc.description.sponsorship | This work was supported by grants from the Anadolu University of Project Department (No. 030326). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Natl Inst Science Communication-Niscair | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Cell Culture | en_US |
dc.subject | Fibronectin | en_US |
dc.subject | Osteosarcoma | en_US |
dc.subject | Type Iv Collagen | en_US |
dc.title | Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis | en_US |
dc.type | article | en_US |
dc.relation.journal | Indian Journal of Experimental Biology | en_US |
dc.contributor.department | Anadolu Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı | en_US |
dc.identifier.volume | 51 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.startpage | 789 | en_US |
dc.identifier.endpage | 796 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.contributor.institutionauthor | Seller, Zerrin | |
dc.contributor.institutionauthor | Sivas, Hülya | |
dc.contributor.institutionauthor | Çiftçi, Gülsen Akalın | |