Comparison of the essential oils of three endemic Turkish Heracleum species obtained by different isolation techniques

Abstract

The essential oils of three Turkish endemic Heradeum species, H. crenatifolium Boiss., H. platytaenium Boiss. and H. sphondylium L. subsp. ternatum (Velen.) Brummitt were obtained using hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD), micro steam distillation-solid-phase microextraction (MSD-SPME)techniques and analyzed by GC and GUMS. Compositions of the oils isolated by different methods showed some differences. The main components in all the oils were found to be octyl acetate (19-95%) and octanol (0.6-5.0%). Octyl isovalerate (0.8-6.4%) and decyl acetate (0.5-4.3%) were the next most abundant constituents in the oil of H. crenatifolium. Octyl hexanoate (3.0-4.7%), (Z)-4-octenyl acetate (1.6-2.1%), octanol (0.7-1.0%) and decanal (0.7-1.2%), were die next major constituents in the oil of H. platytaenium. Octyl butyrate (34.6-41.0%), apiole (4.7-20.4%), (Z)-4-octenyl acetate (2.2-4.4%) and (Z)-4-octenyl butyrate (2.6-3.0%) were found as other main constituents in the oil of H. sphondylium subsp. ternatum.