Gelişmiş Arama

Basit öğe kaydını göster

dc.contributor.authorDikmen, Miriş
dc.date.accessioned2019-10-19T14:16:37Z
dc.date.available2019-10-19T14:16:37Z
dc.date.issued2017
dc.identifier.issn1309-0801
dc.identifier.urihttps://dx.doi.org/10.12991/marupj.259893
dc.identifier.urihttps://hdl.handle.net/11421/13196
dc.descriptionWOS: 000391177500019en_US
dc.description.abstractGlioblastoma multiforme, is the most malignant glioma among other gliomas with high incidence. It has aggresive properties such as high proliferation, invasion and migration rates. Therefore, recently studies have focused on the development of new therapeutic targets in glioblastoma chemotherapy. Substance P, neurokinin-1 (SP/NK-1) receptor system has an important role in cancer development and metastasis. Therefore, NK-1 receptors are new therapeutic targets for glioblastoma therapy and development of NK-1 receptor antagonists. In this study, antiproliferative and apoptotic effects of aprepitant which is an antiemetic drug commonly using in chemotherapy on human U87MG glioblastoma cells were investigated. Antiproliferative effects of aprepitant were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Real Time Analyses System (RTCA DP). Also apoptotic effects of aprepitant were evaluated by annexin-VPI and caspase-3 activity by using flow cytometer. According to the cell index values obtained from real-time cell analysis system, with 50 mu M and higher concentrations, cell proliferation was decreased and IC50 values were determined as 76.9, 62.5 and 59.2 mu M for 24, 48 and 72 hours respectively. Aprepitant showed significant antiproliferative effects on U87MG cells. Depending on increasing aprepitant concentrations, both early and late apoptotic cell ratios were increased. In addition, caspase-3 activation was slightly increased with IC25 and IC50 concentrations of aprepitant according to the control group. As a result, aprepitant showed important antiproliferative and apoptotic effects on U87MG cells. In conclusion, NK-1 receptor antagonists may have a potential therapeutic role for human glioblastoma chemotherapy.en_US
dc.language.isoturen_US
dc.publisherMarmara University, Fac Pharmacyen_US
dc.relation.isversionof10.12991/marupj.259893en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAprepitanten_US
dc.subjectU87Mg Cellen_US
dc.subjectAntiproliferativeen_US
dc.subjectApoptosisen_US
dc.subjectNeurokinin-1 Receptoren_US
dc.titleAntiproliferative and Apoptotic effects of Aprepitant on Human Glioblastoma U87MG Cellsen_US
dc.typearticleen_US
dc.relation.journalMarmara Pharmaceutical Journalen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalıen_US
dc.identifier.volume21en_US
dc.identifier.issue1en_US
dc.identifier.startpage156en_US
dc.identifier.endpage164en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorDikmen, Miriş


Bu öğenin dosyaları:

Thumbnail

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster