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dc.contributor.authorBüyükköroğlu, Gülay
dc.contributor.authorYazan, Emine Yasemin
dc.contributor.authorÖner, Ayşe Filiz
dc.date.accessioned2019-10-19T14:44:00Z
dc.date.available2019-10-19T14:44:00Z
dc.date.issued2015
dc.identifier.issn1300-0527
dc.identifier.urihttps://dx.doi.org/10.3906/kim-1412-40
dc.identifier.urihttps://hdl.handle.net/11421/13345
dc.descriptionWOS: 000362850500009en_US
dc.description.abstractThe aim of this study was to prepare and evaluate stable cationic solid lipid nanoparticles (SLNs) as colloidal carriers for gene therapy. SLNs were mainly composed of three different biocompatible and biodegradable matrix lipids called tripalmitin, glyceryl dibehenate, and triglyceride, all containing the cationic lipid N-(1-(2,3-dioleoyloxy)propy1)-N, N, N-trimethylammonium (DOTAP (R)). Each of these SLNs were divided into three parts and characterized by applying different processes: Part I was autoclaved (121 degrees C, 15 min), Part II was lyophilized (5 mbar, 50 degrees C), and Part III was kept in its intact form. These parts were stored at 4 degrees C, 25 degrees C (room temp.), and 40 degrees C for short-term stability tests. The formulations were tested physically regarding the particle size and zeta potential. pUC18 plasmid DNA was used as the genetic material. Zeta potentials of all SLNs and pDNA-SLN complexes were determined to be highly positive (between +28.90 and +59.39). Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP (R). They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.en_US
dc.description.sponsorshipAnadolu University Research Foundation [020315]en_US
dc.description.sponsorshipThis study was supported by the Anadolu University Research Foundation (Project Number: 020315).en_US
dc.language.isoengen_US
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.isversionof10.3906/kim-1412-40en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCationic Solid Lipid Nanoparticlesen_US
dc.subjectDotap (R)en_US
dc.subjectPlasmid Dnaen_US
dc.subjectGene Deliveryen_US
dc.subjectColloidal Carriersen_US
dc.subjectPreparationen_US
dc.subjectCharacterizationen_US
dc.subjectStabilityen_US
dc.subjectSlnen_US
dc.titlePreparation and physicochemical characterizations of solid lipid nanoparticles containing DOTAP for DNA deliveryen_US
dc.typearticleen_US
dc.relation.journalTurkish Journal of Chemistryen_US
dc.contributor.departmentAnadolu Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji Anabilim Dalıen_US
dc.identifier.volume39en_US
dc.identifier.issue5en_US
dc.identifier.startpage1012en_US
dc.identifier.endpage1024en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorBüyükköroğlu, Gülay


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