Headspace volatiles of Allium subhirsutum L. growing in Turkey

Abstract

Allium subhirsutum L. (Amaryllidaceae family) is a plant species, widely spread around the Mediterranean region from Spain and the Canary Islands to Turkey and Palestine. The A. subhirsutum is called in the regional name 'Tuylu kormen, Koremen' or called 'Yabani sarymsak'. Allium is represented by a single species in Turkey, is a Mediterranean elements. We collected the plant material needed for this study from Marmaris: Yemeler district of Mugla Province. A. subhirsutum sample using headspace - SPME procedure the fibres used in this study were coated with polydimethylsiloxane (PDMS, 100 mu m) (supplied by Supelco Bellefonte, USA), was used with a sampling time of 30 min. Thermal desorption at 250 degrees C during 10 min for the GC/MS analysis. The GC/MS analysis was carried out with an Agilent 5975 GC-MSD system. Innowax FSC column (60 m x 0.25 mm, 0.25 mm film thickness) was used with He as carrier gas. GC oven temperature was kept at 60 degrees C for 10 min and programmed to 220 degrees C at a rate of 4 degrees C/min, and kept constant at 220 degrees C for 10 min and then programmed to 240 degrees C at a rate of 1 degrees C/min, at splitless mode. MS were taken at 70 eV. Mass range was from m/z 35 to 450. Headspace volatiles of crushed bulbs of A. subhirsutum were analyzed by gas chromatography/mass spectrometry. The volatiles were trapped by SPME in a dynamic headspace set up. Volatiles of crushed bulbs of A. subhirsutum were trapped on an HS-SPME (Red fiber). Main components were found to be allyl methyl disulfide (41.0%), diallyl disulfide (20.7%) and dimethyl sulfide (15.3%).