Preparation, characterization and in vitro evaluation of dirithromycin loaded Eudragitr® RS100 nanoparticles for topical application

Abstract

The purpose of the study was to formulate dirithromycin (DR) loaded Eudragit® RS 100 (ERS) nanoparticles for topical application and to analyze the physical and chemical characteristics and in vitro toxicity of the prepared formulations. The nanoparticles of DR with ERS were formulated by spray drying method. The characteristics of nanoparticles were revealed applying scanning electron microscopy, particle size analysis, zeta potential analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The release rate of DR from drug/polymer nanoparticles was investigated as well. Quantitative determination of cytotoxicity of the formulations was investigated on 3T3 mouse embryo fibroblast cell lines. Analyses of results revealed that nano-range sized self-cationic particles with relative spherical morphology were achieved by spray drying method. Nanoparticles displayed slowed release patterns in comparison with the drug powder as expected. With non-toxic properties and extended releases, DR loaded ERS nanoparticles seem to be a promising drug delivery systems for specific applications in the formulation of pharmaceuticals