Mutagenicity Studies of some Substituted Benzylideneaniline Derivatives

Abstract

The aim of this study was to evaluate the mutagenic potential of newly synthesized eight Benzylideneaniline derivatives by using the Salmonella mutagenicity test, using two strains of Salmonella typhimurium carrying frameshift (TA 98) and base-pair substitution (TA 100) mutations. Strains were used in plate incorporation method in the absence and presence of metabolic activation. In a general manner, 4-chloro, 4-methoxy, 4-nitro, 4-methyl, 2 methoxy, 3-nitro derivatives were found to be mutagenic in TA 100 in the absence of metabolic activation. 4-hydroxy benzylidenaniline and 4-bromo benzylidenaniline exhibited direct mutagenicity in TA 98 in the absence of metabolic activation. In the presence of S9 (metabolic activation) mix, 4-methoxy benzylidenaniline, 4-nitro benzylidenaniline, 2-methoxy benzylidenaniline, 3-nitro benzylidenaniline compounds gave mutagenic results in TA 98 and 4-methoxy benzylidenaniline, 2-methoxy benzylidenaniline, 3-nitro benzylidenaniline compounds in TA 100. Mutagenicity of 4 compounds (4-chloro, 4-nitro, 4-methyl, 4-hydroxy) completely disappeared in TA 100 strain by the use of S9 (metabolic activation) mix. Compounds with nitro and methoxy groups exhibited greater mutagenicity in both strains in the presence and absence of metabolic activation.