Nonomuraea jabiensis sp nov., isolated from arid soil

Abstract

A novel actinomycete, strain A4036(T), was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036(T) was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso-diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H-4). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C-16:0 2-OH, iso-C-16:0 and 10-methyl C-17:0. On the basis of 16S rRNA gene sequence similarity studies, strain A4036(T) grouped in the genus Nonomuraea, being most closely related to Nonomuraea angiospora IFO 13155(T) (99.05%), Nonomuraea candida HMC10(T) (98.78%), Nonomuraea kuesteri GW 14-1925(T) (98.49%), Nonomuraea endophytica YIM 65601(T) (98.42%), Nonomuraea maheshkhaliensis 16-5-14(T) (98.40%), Nonomuraea turkmeniaca DSM 43926(T) (98.38%), Nonomuraea helvata IFO 14681(T) (98.29%), Nonomuraea rubra DSM 43768(T) (98.10%) and Nonomuraea salmonea DSM 43678(T) (98.06%). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were <98%. Despite the high 16S rRNA gene sequence similarities, DNA DNA relatedness values and phenotypic data demonstrated that strain A4036(T) was clearly distinguished from all closely related species of the genus Nonomuraea. Thus, this isolate is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036(T) (=DSM 45507(T)=KCTC 19870(T)).