Effect of Bacillus sp. isolated from the soil against some Fusarium strains

Abstract

In this study a total of 16 Bacillus strains isolated from the some soil examples in Turkey. Also fungi were isolated from diseased sugar beets by using Rose Bengal Chloramphenicol (RBC) Agar and incubated at 25°C for 5-7 days. Identification of grown isolated bacteria colonies was based on morphologic, biochemical and culturing characteristics. However, macroscopic and microscopic properties of the fungi isolates are examined and they were identified microscopically to genus level with traditional methods using diagnostic literatures. Antifungal activity of isolated bacteria strains were determined against Fusarium oxysporum, Fusarium solani, Fusarium culmorum, Fusarium moniliforme standard strains and our 2 Fusarium sp. isolates. Isolated 16 bacteria strains were Gram positive rods and were identified as Bacillus sp. and our 2 fungal isolates were identified as Fusarium sp. As a result of antimicrobial activity studies, 6 Bacillus spp. isolates had antibacterial activity against at least two or more Fusarium strains. The inhibition zones ranged between 1.5±0.000-7.6±0.000 cm