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dc.contributor.authorSay, Rıdvan
dc.contributor.authorSenay, R. Hilal
dc.contributor.authorBiçen, Özlem
dc.contributor.authorErsöz, Arzu
dc.contributor.authorYılmaz, Filiz Şişman
dc.contributor.authorAkgol, Sinan
dc.contributor.authorDenizli, Adil
dc.descriptionWOS: 000317528700009en_US
dc.descriptionPubMed ID: 23498211en_US
dc.description.abstractalpha-Amylase (EC; alpha-D-1,4,glucan glucanohydrolase) catalyzes the hydrolysis of alpha-D-(1,4)-glucosidic linkages in starch, glycogen, and various malto-oligosaccharides, by releasing alpha-anomeric products. In this study, a novel method has been developed to prepare nanoprotein particles that carry alpha-amylase as a monomer by using a photosensitive microemulsion polymerization process. The nanostructured alpha-amylase with photosensitive features have been characterized by fluorescence spectroscopy, transmission electron microscopy (TEM) and Zeta Sizer. The fluorescence intensity of amylase nanoparticles was determined to be 658 a.u. at 610 nm and the average particle size of nanoamylase was found to be about 71.8 nm. Both free alpha-amylase and nanoparticles were used in the hydrolysis of starch under varying reaction conditions such as pH and temperature that affect enzyme activity and the results were compared to each other. Km values were 026 and 0.87 mM and V-max values were 0.36 IU mg(-1) and 22.32 IU mg(-1) for nanoenzyme and free enzyme, respectively. Then, thermal stability, storage stability and reusability were investigated and according to the results, activity was preserved 60% at 60 degrees C; 20% at 70-80 degrees C temperature values and 80% after 105 days storage. Finally after 10 cycles, the activity was preserved 90% and this novel enzymatic polymeric amylase nanoparticle has showed considerable potential as reusable catalysten_US
dc.publisherElsevier Science BVen_US
dc.subjectPhotosensitive Microemulsionen_US
dc.subjectPhotosensitive Cross-Linkingen_US
dc.subjectProteinous Polymeric Particlesen_US
dc.titlePolymeric amylase nanoparticles as a new semi-synthetic enzyme system for hydrolysis of starchen_US
dc.relation.journalMaterials Science & Engineering C-Materials For Biological Applicationsen_US
dc.contributor.departmentAnadolu Üniversitesi, Fen Fakültesi, Fizik Bölümüen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorSay, Rıdvan
dc.contributor.institutionauthorErsöz, Arzu

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