Determination of amoxicillin by flow injection analysis using UV-detection, potentiometry, and conductometry in pharmaceutical preparations

Abstract

A method for direct determination of amoxicillin in pharmaceuticals using flow injection analysis (FIA) with UV detection adjusted at 228 nm, along with potentiometer and condoptometric methods are described. The best carrier solvent system consisted of an aqueous solution of methanol (10% v/v) with pH 9 pumped at a flow rate of 1 mL . min(-1). The limit of detection (LOD) and quantification (LOQ) for FIA was calculated to be 3.3 x 10(-7) M (S/N = 3) and 1.0 X 10(-7) M (S/ N = 10), respectively. Two pharmaceutical formulations containing amoxicillin were studied. In the analysis of the tablets, the RSD values were found to be 1.36 and 1.21 and 1.18 (for AMOKLAVIN (R)), 1.10 1.16 and 1.14 (for REMOXIL (R)) for FIA, potentiometer, and conduct metric methods, respectively. The proposed methods were applied to the pharmaceutical tablets and good results were obtained. These proposed methods are simple, fast, accurate, precise, and cost effective for the routine determination of amoxicillin in pharmaceutical preparations.